Capturing circulating tumor cells (CTCs) and/or circulating tumor DNA from blood, which represents a precious source of biological material derived from both primary and metastatic tumors, has been named a 'liquid biopsy'. While the circulating tumor DNA might be more representative of the bulk of the metastatic tumor, CTCs are thought to reflect more of the metastases-initiating cells. Consequently, a liquid biopsy made of tumor cells and tumor DNA that is able to track cancer evolution, as a fingerprint of the patient's individual tumor, and is easy to perform at every stage of the disease course, sounds attractive. This article mainly focuses on the applications of CTCs to track tumor dynamics in real time using colorectal cancer as a model system. The analysis of viable CTCs at DNA, RNA and protein levels, as well as their expansion in vitro, may allow deep investigation of the features of metastases-initiating cells.

The rationale for liquid biopsy in colorectal cancer: a focus on circulating tumor cells / Gazzaniga, Paola; Raimondi, Cristina; Nicolazzo, Chiara; Carletti, Raffaella; DI GIOIA, Cira Rosaria Tiziana; Gradilone, Angela; Cortesi, Enrico. - In: EXPERT REVIEW OF MOLECULAR DIAGNOSTICS. - ISSN 1473-7159. - STAMPA. - 15:7(2015), pp. 925-932. [10.1586/14737159.2015.1045491]

The rationale for liquid biopsy in colorectal cancer: a focus on circulating tumor cells

Paola Gazzaniga
Primo
;
Cristina Raimondi
Secondo
;
Chiara Nicolazzo;Raffaella Carletti;Cira di Gioia;Angela Gradilone
Penultimo
;
Enrico Cortesi
Ultimo
2015

Abstract

Capturing circulating tumor cells (CTCs) and/or circulating tumor DNA from blood, which represents a precious source of biological material derived from both primary and metastatic tumors, has been named a 'liquid biopsy'. While the circulating tumor DNA might be more representative of the bulk of the metastatic tumor, CTCs are thought to reflect more of the metastases-initiating cells. Consequently, a liquid biopsy made of tumor cells and tumor DNA that is able to track cancer evolution, as a fingerprint of the patient's individual tumor, and is easy to perform at every stage of the disease course, sounds attractive. This article mainly focuses on the applications of CTCs to track tumor dynamics in real time using colorectal cancer as a model system. The analysis of viable CTCs at DNA, RNA and protein levels, as well as their expansion in vitro, may allow deep investigation of the features of metastases-initiating cells.
2015
cancer stem cells; circulating tumor cells; colorectal cancer; epithelial-mesenchymal transition; liquid biopsy; targeted therapy; biopsy; colorectal neoplasms; epithelial-mesenchymal transition; humans; molecular targeted therapy; neoplasm metastasis; neoplastic cells, circulating; neoplastic stem cells; 2734; molecular medicine; molecular biology; genetics
01 Pubblicazione su rivista::01g Articolo di rassegna (Review)
The rationale for liquid biopsy in colorectal cancer: a focus on circulating tumor cells / Gazzaniga, Paola; Raimondi, Cristina; Nicolazzo, Chiara; Carletti, Raffaella; DI GIOIA, Cira Rosaria Tiziana; Gradilone, Angela; Cortesi, Enrico. - In: EXPERT REVIEW OF MOLECULAR DIAGNOSTICS. - ISSN 1473-7159. - STAMPA. - 15:7(2015), pp. 925-932. [10.1586/14737159.2015.1045491]
File allegati a questo prodotto
File Dimensione Formato  
Gazzaniga_Rationale-liquid_2015.pdf

solo gestori archivio

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 413.51 kB
Formato Adobe PDF
413.51 kB Adobe PDF   Contatta l'autore

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/782142
Citazioni
  • ???jsp.display-item.citation.pmc??? 10
  • Scopus 15
  • ???jsp.display-item.citation.isi??? 16
social impact